Fig. 6: Mutations in SD1 lead to loss of neutralizing activity.
From: The SARS-CoV-2 neutralizing antibody response to SD1 and its evasion by BA.2.86
a Mutations found in SD1 together with their incidence, the most prevalent variants, their first recorded submission, the percentage of sequence reports for each mutation in the last 2 months, and peak time of submission. b Front (left panel) and back (right panel) views of the SD1 domain are shown as surface representations with the SD1-1 footprint colored in pale green and mutation sites from a highlighted in different colors. c Neutralization assays using indicated pseudoviral constructs with mAbs SD1-1, 2, 3, IC50 values are shown in Supplementary Table 4. n = 2 independent experiments with duplicates. Data are presented as mean values ± SEM. d E554K submitted sequences over time. e Residue L560 packs against E224 from NTD of a neighboring chain, it does not have any direct contact with any of the three SD1 Fabs reported here except its proceeding residue F559 H-binds to Y31 of SD1-3 LC. f Interactions of E554 with SD1-1, 2, and 3, which are potentially interrupted by E554K mutation (semi-transparent green sticks) in BA.2.86. g Effect of K535R on SD1-1 and SD1-3. The drawing and color schemes are as in Fig. 4e. Source data are provided as a Source Data file.
