Fig. 4: Efficient meiotic recombination relies on IHO1-HORMAD1 interaction.
From: Seeding the meiotic DNA break machinery and initiating recombination on chromosome axes
A Radiograph of immunoprecipitated and radioactively labeled SPO11-oligo complexes from testes of 13 dpp juvenile mice. Bar, SPO11-specific signals, asterisk, nonspecific labelling, and arrowhead, immunoglobulin heavy-chain. Radioactive signals were background-corrected (Iho1−/−, signal=0) and normalized to corresponding wild type control (1). B Quantification of SPO11-oligo complexes from 13dpp mice. Bars are mean, n=number of mice. Two-tailed paired t-test, ns=P = 0.3419. C–E Immunostaining in nuclear spread early zygotene spermatocytes of adult mice. Bars, 10 µm. F, G Quantification of axis associated DMC1 (F), RPA2 (G) focus numbers in spermatocytes. Pools of two experiments are shown (one mouse represented each genotype in each experiment). Bars are medians, n=cell numbers. Two-tailed Mann Whitney U-test, ns=P > 0.05, *=P < 0.05, **=P < 0.01, ****=P < 0.0001. Exact P values: (F), leptotene, wild type vs. Iho1C7Δ/C7Δ, P = 0.05, Iho1C7Δ/C7Δ vs. Hormad1−/−, P = 0.486, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.234, early zygotene, wild type vs. Iho1C7Δ/C7Δ, P = 1.26e-7, Iho1C7Δ/C7Δ vs. Hormad1−/−, P = 7.38e-6, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.984, late zygotene, wild type vs. Iho1C7Δ/C7Δ, P = 9.44e-9, Iho1C7Δ/C7Δ vs. Hormad1−/−, P = 1.61e-6, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.373, pachytene, wild type vs. Iho1C7Δ/C7Δ, P = 1.5e-6, (G), leptotene, wild type vs. Iho1C7Δ/C7Δ, P = 0.02417, Iho1C7Δ/C7Δ vs. Hormad1−/−, P = 0.01189, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.2825, early zygotene, wild type vs. Iho1C7Δ/C7Δ, P = 2.19e-9, Iho1C7Δ/C7Δ vs. Hormad1−/−, P = 3.17e-8, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.2455, late zygotene, wild type vs. Iho1C7Δ/C7Δ, P < 2.2e-16, Iho1C7Δ/C7Δ vs. Hormad1−/−, P < 2.2e-16, Hormad1−/− vs. Hormad1−/− Iho1C7Δ/C7Δ, P = 0.4054, pachytene, wild type vs. Iho1C7Δ/C7Δ, P = 7.28e-15. See also related Supplementary Fig. 7. Source data are provided as a Source Data file.
