Fig. 4: α-Synuclein inclusion-bearing neurons show gene expression changes associated with cellular dysfunction.

a A heatmap displaying the z-score of all genes differentially expressed between pSyn- and pSyn+ segments with FDR-corrected p-value < 0.01. Both rows and columns have been sorted by unsupervised clustering. (n = 11 mice) b Volcano plot comparing genes differentially expressed enriched in pSyn- segments (blue) and pSyn+ segments (red) across regions as determined by two tailed mixed effects linear regression with multiple test correction using the FDR. c Examples of 25 genes that are differentially expressed in pSyn- and pSyn+ segments that fit into one of 9 categories as noted by the color and corresponding symbol. From top-to-bottom, left-to-right, categories are synapse, mitochondria, lysosome, ubiquitin-proteasome, DNA damage-apoptosis, cytoskeleton, complement-cytokine. d Example pathways that are disrupted in LB-bearing neurons with relative gene expression plotted by color (blue: enriched in pSyn-, red: enriched in pSyn+) for each gene in the 3 pathways.