Fig. 6: α-Synuclein pathology is enriched in layer 5 intratelencephalic and layer 6b neurons.

a Cell abundance estimates from cell deconvolution analysis in layer 5 of MOs show enrichment of L5 PT, Pvalb, and Sst neurons in pSyn- segments and enrichment of L5 IT neurons in the pSyn+ neurons (n = 11 mice). b Cell abundance estimates from cell deconvolution analysis in layer 6 of MOs (n = 11 mice). show enrichment of Sncg cell types in pSyn- neurons and an enrichment of L6b neurons in pSyn+ neurons. Each dot is an individual segment and FDR-corrected p-values are displayed for statistical comparison. c A zoomed in view of layer 5 of MOs with yellow arrowheads denoting neurons with pSyn inclusions. Scale bar = 50 µm. d Examples of single cells positive for Slc17a7, Gad1, and/or pSyn. Scale bar = 10 µm. e Percentage of pSyn inclusions in cortical layers as a percentage of the total inclusions. f Percentage of cells with pSyn inclusions also positive for either Slc17a7 or Gad1 in each cortical layer. p-values are displayed from a mixed-effects analysis with Sidak’s multiple comparisons test to compare Slc17a7 and Gad1 positive neurons. g A zoomed in view of layer 5 of MOs with yellow arrowheads denoting neurons with pSyn inclusions. Scale bar = 50 µm. h Examples of single cells positive for Pcp4, Rorb, and/or pSyn. Scale bar = 10 µm. i Percentage of cells with pSyn inclusions also positive for Rorb in each cortical layer. j Quantification of the percentage of cells with pSyn inclusions also positive for Pcp4 in each cortical layer. k A zoomed in view of layer 5 of MOs with yellow arrowheads denoting neurons with pSyn inclusions. Scale bar = 50 µm. l Examples of single cells positive for Lamp5 and/or pSyn. Scale bar = 10 µm. m Percentage of cells with pSyn inclusions also positive for Lamp5 in each cortical layer. Data in panels e, f, i, j, and m are presented as mean values +/- SEM with values for individual mice plotted (n = 12 mice). Source data are provided as a Source Data file.