Fig. 1: Knockdown of condensin II subunit Cap-d3 results in smaller Drosophila adult heads and brains. | Nature Communications

Fig. 1: Knockdown of condensin II subunit Cap-d3 results in smaller Drosophila adult heads and brains.

From: Condensin-mediated restriction of retrotransposable elements facilitates brain development in Drosophila melanogaster

Fig. 1

a qRT-PCR analyses of cap-d3 transcripts were performed on cDNA generated from first instar larvae expressing control UAS-GFP dsRNA (pink) or UAS-cap-d3 dsRNA (red) under the control of armGAL4. Transcripts were normalized to the housekeeping gene, rp49. The chart shown includes technical replicates and is representative of two independent experiments. P values were determined by performing a two-tailed, unpaired t-test. b Adult female fly heads were dissected from flies expressing control UAS-GFP dsRNA (top row) or UAS-cap-d3 dsRNA (bottom row) under the control of eyGAL4, GMRGAL4. c–e The distance between two machrochaetes positioned between the eyes of adult flies was measured as a proxy for measuring adult head size. The region measured is shown in (c). Adult, female fly head sizes were measured on flies expressing control UAS-GFP dsRNA (pink) or UAS-cap-d3 dsRNA (red) driven by eyGAL4, GMRGAL4 at 25 °C (d) or at 18 °C (e). Charts shown in (d) and (e) are representative of two independent experiments; each data point represents a single fly. f Adult brain volumes were measured in female flies (1–3 days post-eclosure) expressing control UAS-GFP dsRNA (pink) or UAS-cap-d3 dsRNA (red) driven by eyGAL4, GMRGAL4 at 25 °C. The charts shown are representative of two independent experiments; each data point represents a single brain. g Adult head sizes were measured in female flies expressing different combinations of control UAS-GFP dsRNA or UAS-cap-d3 dsRNA and UAS-GFP or UAS-GFP-cap-d3 under the control of eyGAL4, GMRGAL4. The results shown are the combined results of two independent experiments; each data point represents a single fly. For experiments in (d–g), P values were determined by performing two-tailed Mann–Whitney analyses. For a, p ≤ 0.0001. For d, p = 0.0001. For e, p = 0.0121. For f, p = 0.0010. For g, **p = 0.0060, ***p = 0.0001, ****p ≤ 0.0001. NS not significant. For all experiments, error bars indicate standard deviations from the mean.

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