Fig. 4: Cap-d3 knockdown in the developing central brain reduces adult brain volumes. | Nature Communications

Fig. 4: Cap-d3 knockdown in the developing central brain reduces adult brain volumes.

From: Condensin-mediated restriction of retrotransposable elements facilitates brain development in Drosophila melanogaster

Fig. 4

a–c Third instar larval brains were dissected from male larvae expressing UAS-GFP under the control of a eyGAL4, GMRGAL4, b optixGAL4, or c c253GAL4, immunostained with antibodies to detect GFP (green) and stem cell marker, Dpn (red), and imaged using confocal microscopy. Maximum projections of z-stacks are shown. Nuclei are stained with DAPI (blue). All images were taken with 40x magnification. All images shown are representative of at least five biological replicates. Scale bar = 50 µm. d, e Adult brain volumes were measured in male flies expressing control UAS-GFP dsRNA or UAS-cap-d3 dsRNA driven by d optixGAL4 or e c253GAL4 at 25 °C; each data point represents a single brain. P values were determined by performing two-tailed Mann–Whitney analyses. For (d), p = 0.0156. For (e), p = 0.0278. Error bars indicate standard deviations from the mean. Results shown include adults harvested from two independent experiments.

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