Fig. 5: Auto-inhibition of the γ-subunit.
From: Architecture and activation of human muscle phosphorylase kinase
a Structural overlay of human PhKγ and rabbit PhKγ (PDB ID: 2PHK). Lys361γ and Gly362γ occupy the –3 and –2 sites of the substrate peptide (MC-peptide) in rabbit PhKγ. b The αγδ subcomplex and PhK holoenzyme containing the K361A/Q363A/R367A (3M) mutations in the γ-subunit, as well as γ326δ, all display Ca2+-independent kinase activity. This experiment has been repeated three times.
