Fig. 2: Quality assessment of scCAMERA.

A Top: Summary of the accuracy of cell annotation clarity using cell-specific reporters. Bottom: An example illustrating the overlap of a reporter known to be expressed in the ASEL cell with mir-124p::mNG::H2B annotated as expressed in the ASEL cell. B Comparison of miRNA reporter expression with global miRNA-seq results. Left: Heatmap showing the relative expression of each miRNA analyzed in this study versus four previous studies using miRNA-seq. miRNAs are classified as high, medium, and low expression based on the number of miRNA-pr expression cells observed in this study. Right: Comparison of miRNA-seq expression values among miRNAs classified into high (H), medium (M), and low (L) expression in corresponding miRNA-seq datasets. The data are summarized as boxplots with boxes indicating the inter-quartile range (IQR), whiskers showing the range of values within 1.5*IQR, and horizontal lines indicating medians. Statistics: Mann–Whitney U test, two-tailed. C Comparison of miRNA reporter expression with tissue-level miRNA-seq data. Data organization and statistics remain consistent with those presented in (B). D Comparison of cellular fluorescence intensity of miRNA reporters with endogenous fluorescent knockin (KI) reporters. Each image is a maximum projection of 3D fluorescence images acquired using the laser power (expressed as a percentage of the maximum) listed on the right. Source data are provided as a Source Data file.