Fig. 3: Fine patterning of cells by combinatorial expression of miRNAs.

A Distribution of tissue types (columns) of cells in each cell cluster (row). Different colors indicate the percentage of cells in each cell cluster. Only cell clusters with more than one cell are shown. B Top: Schematic representation of the anatomy of the rectum-anus. Bottom: Cell cluster (box) distribution of cell types (oval) constituting the rectum-anus. Numbers in ovals indicate cell number, and the thickness of lines connecting ovals and boxes indicate the percentage of cells within the type that is classified into a given cluster. C Schematic representation of the position of six rectum epithelial cells relative to the intestinal-rectal valve. D Representative miRNA-prs that exhibited selective expression enrichment in K and K’ cells among all six epithelial cells. E Pan- and sub-tissue-specific miRNA-prs identified in embryonic and L1 stages. R & A indicates rectum-anus. F Single-cell, spatiotemporal expression of representative pan-tissue miRNA-prs. For each miRNA-pr, the expression visualization is identical to Fig. 1G. The set of barcodes at the bottom illustrates embryonic and L1 expression in cells ordered by tissue type, with the black box highlighting the enriched tissue type. 3D rendering showing sub-tissue-specificity of representative miRNAs in the pharynx (G) and rectum-anus (H) at the L1 stage. Blue highlights the cell location of a specific cell type (indicated on the left). I Summary of the results of comparing scCAMERA to the literature. J Heatmap illustrating the detection of tissue enrichment for miRNA-prs identified in this study, compared with findings from previous studies. K Frequency of each type of tissue- and sub-tissue-specific TF-pr (top) and miRNA-pr (bottom). Statistics: one-sided hypergeometric test.