Fig. 5: Branched N-glycans increase the binding affinity of PD-L1 on HR-proficient cancer cells to PD-1 on CD8⁺ T cells.

a Frequency of PHA-L binding to HR-proficient cells treated with 1 mM 2DG for 48 h. P-values were calculated using two-tailed t-test. n = 3 biologically independent samples. Error bars represent mean with SD. b Frequency of PHA-E binding to HR-proficient cell OVCAR3 treated with 1 mM 2DG for 48 h. P-values were calculated using two-tailed t-test. n = 3 biologically independent samples. Error bars represent mean with SD. c Frequency of recombinant PD-1 binding to PD-L1⁺ HR-proficient cells treated with 1 mM 2DG for 48 h. P-values were calculated using two-tailed t-test. n = 3 biologically independent samples. Error bars represent mean with SD. d Reverse correlation between MGAT5 mRNA expression and tumor-infiltrating activated CD8⁺ T cells in the TCGA HGSOC dataset (two-tailed Spearman’s r correlation test), data was analyzed by TISIDB. e Killing of HR-proficient PEO4 cells expressing CD19 was measured after coculture with anti-CD19 CAR T cells at the 1:6 E:T ratio. n = 3 biologically independent samples. f Killing of HR-proficient OVCAR3 cells expressing CD19 was measured after coculture with anti-CD19 CAR T cells at the 1:6 E:T ratio. n = 3 biologically independent samples. g Killing of HR-deficient PEO1 cells expressing CD19 was measured after coculture with anti-CD19 CAR T cells at the 1:6 E:T ratio. n = 3 biologically independent samples. Two-tailed P-values were calculated using ANOVA used for analyses and then corrected using the Benjamini, Krieger, and Yekutieli method to generate q-values unless otherwise stated. Error bars represent mean with SEM. Source data are provided as a Source Data file.