Fig. 2: Sub-cellular localization of SA nanosensor in living plants.
From: Decoding early stress signaling waves in living plants using nanosensor multiplexing
Confocal images of tobacco leaf infiltrated with SA sensor to visualize the subcellular localization of SA sensor: a, e, j Chlorophyll autofluorescence (red), b, f, k SA sensor Cyan florescence, c, g, l overlay and (d, h, m) brightfield. Row 1—SA fluorescence was observed in epidermal cell periphery indicating cytoplasmic localization. Row 2—plasmolyzed epidermal cells (with 0.8 M Mannitol), showing SA sensor fluorescence in the apoplastic space formed by the shrinking protoplast as indicated by the arrows. i Zoom-in overlay image of a cell from Fig. 2g with corresponding brightfield image area marked with dotted cyan box in Fig. 2h, asterisk indicate the apoplastic space. Row 3—overlap of red chlorophyll autofluorescence with cyan SA fluorescence in mesophyll cells indicating chloroplast localization of SA sensor. Row 1, Row 2, and Row 3 Images are representative of at least three independent experiments.
