Fig. 5: FOXO1 SIM as a Nuclear Exit Signal for FOXO3.
From: Cold-induced FOXO1 nuclear transport aids cold survival and tissue storage
a Schema of FOXO3 mutant construct that contains the FOXO1 SIM. b Confocal images of FOXO3 and DAPI staining in ARPE-19(WT) cells at indicated conditions (n = 6 experiments). c Confocal images of FOXO3, HA tag and DAPI staining in ARPE-19 cells overexpressing FOXO3-plus SIM-HA at indicated conditions. d FFOXO3 ratio (from left to right, n = 10, 14, 15 and 15 images from 5 experiments). e A model for temperature and FOXO1 SIM-mediated FOXO1 transportation. The illustration was based on a template created with BioRender.com. Data are shown as mean and SEM. Statistics: two-tailed Student’s t test (d). Scale bars: 20 μm (b, c). Source data are provided as a Source Data file.
