Fig. 2: ChIP-Seq analysis of H-NS binding to Salmonella chromosomal DNA. | Nature Communications

Fig. 2: ChIP-Seq analysis of H-NS binding to Salmonella chromosomal DNA.

From: Transcription-driven DNA supercoiling counteracts H-NS-mediated gene silencing in bacterial chromatin

Fig. 2

a Representative ChIP-Seq profiles from strain MA14443 (∆[sitA-prgH1.2]::tetRA-ThisL hns-3xFLAG) grown to early stationary phase in the absence or in the presence of 0.4 µg/ml AHTc. b Read count quantification in selected genome sections. Read counts within the sections highlighted and numbered in (a) and in the H-NS-bound regions of the indicated chromosomal loci were determined with the “Samtools’ view” tool and normalized to the total reads from the entire genome. The histogram bars represent the ratios between the normalized values from IP samples and those from input DNA. The data represent the means from three independent ChIP-Seq experiments (each identified by the round symbol color, with error bars indicating standard deviations). Source data are provided as a Source Data file.

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