Fig. 1: Osmotically driven scission of DM and SM tubes.

Time sequence of SM (a) and DM tube scission (b) upon constriction by osmotic stress. The membrane is shown in pseudocolor. Cartoons illustrate the steps in the scission process in each case, while stars mark scission events. See also Supplementary Movie 1. c Quantification of the radii of the SM and the inner membrane of the DM tubes right before scission (n = 3 independent experiments; each point represents a tube). Inner DM radii were estimated assuming a constant intermembrane (IM) distance between opposing hydrophobic heads region as 2 or 5 nm, respectively (see “Methods”). ** statistically different at the 0.01 level; *** statistically different at the 0.001 level (unpaired two-sided two sample t-test, equal variance assumed; Box plots indicate median (middle line), 25th and 75th percentile (box), and outliers (whiskers)). Source data are provided as a Source data file.