Fig. 3: PANX1 pore substitution mutant, PANX1WR/RD constricts the channel.
From: Cryo-EM structures of pannexin 1 and 3 reveal differences among pannexin isoforms
a The superposition of PANX1 (blue) and PANX1WR/RD (violet) illustrates that in PANX1WR/RD, R74 and D75 serve as pore residues, contrasting with W74 and R75 in PANX1WT. b A cross-section of superposed PANX1WT and PANX1WR/RD displays the reduction of pore. c The superposition of PANX1WR/RD and PANX2 unveils the pore residues in PANX2, with the density for R74 and D75 in PANX1WR/RD contoured at 9.0 σ. d A cross-section of superposed PANX2 and PANX1WR/RD displays the position of arginine at the pore. The distances depicted in the figure are in angstroms (Å). e, f The hole profile displays similar pore radius for PANX1WR/RD and PANX2, The line represents the minimum radius for PANX2 at 2.7 Å formed by the first constriction (R89) in PANX2 channel. Units of both X and Y-axes are in angstroms (Å). g The binding affinity for the PANX1WR/RD was determined to be 67 ± 17 µM, n = 3 independent experiments, error bar represents S.D.
