Fig. 1: SopB mediates the disassembly of RAB10⁺ tubules and delivery of RAB10 to STm invasion sites.

a Current model of SopB-mediated RAB10⁺ vesicle recruitment to invasion sites. b Representative live images of RAB10⁺ tubules in WT Henle 407 cells, pre-infection and at indicated time points post-WT BFP-STm (in red) infection (p.i.). RFP-LifeAct and BFP-STm were used to identify invasion sites in real-time. Data are representative of three independent experiments. c, d Representative images (c) and quantifications (d) of RAB10⁺ tubules and RAB10⁺ invasion sites in either pre-infection or WT STm-infected Henle 407 cells for the indicated time. RFP-LifeAct and BFP-STm were used to identify invasion sites in real-time. Inset ‘i’ indicates an ROI with RAB10⁺ tubules while inset ‘ii’ indicates an ROI of a representative invasion site. n = 3 Independent experiments. At least 100 cells for each condition in each experiment were scored for the presence or absence of RAB10-positive membrane reservoirs or 100 invasion sites were scored for RAB10 recruitment. e, f Representative images (e) and quantifications (f) of RAB10⁺ tubules and RAB10⁺ invasion sites in Henle 407 cells pre-infection and at indicated time points post ΔsopB STm infection. RFP-LifeAct and BFP-STm were used to identify invasion sites in real-time. Inset ‘i’ indicates an ROI with RAB10⁺ tubules while inset ‘ii’ indicates an ROI of a representative invasion site. n = 3 Independent experiments. At least 100 cells for each condition in each experiment were scored for the presence or absence of RAB10-positive membrane reservoirs or 100 invasion sites were scored for RAB10 recruitment. Data shown are means ± standard deviation (SD). P value was calculated using (d, f) two-way ANOVA with Tukey’s HSD post-hoc test. Scale bars, b 8 μm, c and e 5 μm. Source data are provided as a Source Data file.