Fig. 5: Exocyst subunits EXOC2 and EXOC3 are independently recruited to STm invasion sites.

a Representative images of WT Henle 407 cells transfected with GFP-EXOC3 and RFP-EXOC2 constructs and infected with WT STm. Cells were fixed and imaged at 10 min post-infection. In this and the following panels, invasion sites were identified by actin and STm staining. Data are representative of three independent experiments. b WT Henle 407 cells were transfected with GFP-EXOC2 and myc-PM-RAB10 (upper panel) or GFP-EXOC3 (lower panel) and then fixed and stained for myc-tag (upper panel) or GM130 (lower panel). Representative images and line plot profiles (white arrows in insets) depicting the localization of EXOC2 on RAB10⁺ tubular membrane reservoirs and EXOC3 on Golgi membrane. MCC values for GFP-EXOC2 and myc-PM-RAB10 are M1 = 0.46 ± 0.08 and M2 = 0.44 ± 0.07. For GFP-EXOC3 and GM130, M1 = 0.24 ± 0.06 and M2 = 0.61 ± 0.07. Data are representative of three independent experiments. c Quantifications of the percentage of cells with EXOC2⁺ tubules, in WT, RAB10 KO or RAB10 KO Henle 407 cells with myc-PM-RAB10 overexpression. n = 3 Independent experiments with 100 cells examined in each experiment. d, e Representative images (d) and quantifications (e) of EXOC2 recruitment to STm invasion sites. WT, RAB10 KO or EXOC3 KO Henle 407 cells were transfected with GFP-EXOC2 and then infected with WT STm. Cells were fixed and imaged at 10 min post-infection. n = 3 Independent experiments with 100 invasion sites examined in each experiment. f Quantifications of EXOC3 recruitment to STm invasion sites. WT, RAB10 KO or EXOC2 KO Henle 407 cells were transfected with GFP-EXOC3 and then infected with WT STm. Cells were fixed and imaged at 10 min post-infection. n = 3 Independent experiments with 100 invasion sites examined in each experiment. Data shown are means ± SD. P value was calculated using one-way ANOVA. Scale bars, 10 μm. Source data are provided as a Source Data file.