Fig. 7: Mobilization of membrane reservoirs promotes STm invasion.

a Representative SEM image of invasion ruffles. WT Henle 407 cells and indicated knockout Henle 407 cells were infected with WT STm and fixed at 10 min post-infection. b Quantifications of the area of invasion ruffles identified as in (a). The areas of individual invasion ruffle were measured as described in “Methods”. n = 3 Independent experiments with 15 invasion ruffles examined in each experiment. c Representative images of invasion ruffles at indicated time points WT STm post-infection. WT and RAB10 KO Henle 407 cells were transfected with GFP-LifeAct and infected with WT BFP-STm. GFP-LifeAct and BFP-STm were used to identify invasion sites. d Quantifications of the volume of invasion ruffles are identified as in (c). The volumes of individual invasion ruffles were measured as described in “Methods”. n = 3 Independent experiments with ten invasion ruffles examined in each experiment. e WT Henle 407 cells and indicated knockout Henle 407 cells were infected with WT STm and lysed at 2 h post-infection for CFU counting, n = 3. f Model depicting how STm exploits RAB10⁺ membrane reservoirs for the invasion of host cells. Data shown are means ± SD. P value was calculated using (b, e) one-way ANOVA. Scale bars, 10 μm. Source data are provided as a Source Data file.