Fig. 2: Irradiation Treatment Sample Collection and Antibody Panel. | Nature Communications

Fig. 2: Irradiation Treatment Sample Collection and Antibody Panel.

From: Microenvironmental reorganization in brain tumors following radiotherapy and recurrence revealed by hyperplexed immunofluorescence imaging

Fig. 2

a PDGfp and BrM tumors were initiated in NTVA-Ink4a/Arf−/− and C57Bl6 mice respectively, and monitored by MRI. Experimental endpoints (red arrows) are indicated for each group. Biweekly MRI monitoring tracked the process of regression and recurrence for each mouse following IR for b, c PDGfp (n = 5 mice for each treatment group) and d, e BrM tumors (n = 3 mice for each treatment group. Upon tumor detection, mice were grouped into cohorts and collected as either (i) ‘untreated’ samples, (ii) treated with 10 Gy (PDGfp) or 15 Gy (BrM) focalized irradiation (IR) therapy and harvested 7 days post-IR, or (iii) treated with 10 or 15 Gy IR and harvested upon tumor regrowth. White arrows indicate the tumor in both tumor types, red arrows indicate post-IR lesion in PDGfp tumors. Source data are provided as a Source data file.

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