Fig. 1: Phage cocktail reduces burden of intestinal VRE. | Nature Communications

Fig. 1: Phage cocktail reduces burden of intestinal VRE.

From: Phage-specific immunity impairs efficacy of bacteriophage targeting Vancomycin Resistant Enterococcus in a murine model

Fig. 1

Five lytic bacteriophages were chosen for use in a therapeutic cocktail. A Spot titer of bacteriophage lysates on a VRE bacterial lawn. B Transmission electron microscopy of each of the five phages used in cocktail. Line displayed represents 100 nm. C Schematic of mouse model of phage therapy targeting colonized VRE. D C57Bl/6 J mice were given antibiotics for 1 week prior to inoculation with 108 CFU of VRE via oral gavage. Mice were treated with daily intraperitoneal injections (IP) with either a five-phage cocktail (ɸCT) (109 PFU/phage) or vehicle control for 7 days. Stool was plated to quantify VRE in colony-forming units (CFU) per gram shown with geometric mean ± standard deviation. Data are representative of three experiments with n = 5 per group. Fischer’s mixed-effects uncorrected LSD test was used between groups. Significance indicated by *p < 0.01, **p < 0.001. Source data are provided as a Source Data file.

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