Fig. 2: Antibody production in response to administration of bacteriophage cocktail.
Mice were treated with either ɸCT or vehicle control for 7 days, allowed to rest for 1 month, and then treated with the same phage regimen a second time. Serum was collected 1 day after completion of the secondary course (day 35). A IgG anti-phage antibodies were detected with ELISA. Mean ± SD with sigmoidal curve-fit is shown. B Serum titer was calculated using IC50 from sigmoidal curves in A. One-way ANOVA with Holm-Å Ãdák correction was performed. C IgM anti-phage antibodies (D) Serum (diluted 1:10) from mice was incubated with purified phages (109 PFU/mL) to test for neutralization of phage killing activity. Efficiency of plating (EOP) was determined by dividing titers from phage incubated with serum by phage incubated with SM buffer alone. EOP limit of detection (LOD) is shown which was calculated by dividing titers of LOD and SM buffer alone. Two-way ANOVA with Holm–ŠÃdák correction used. E Schematic of mouse model. F Mice were treated with a primary course of either phage cocktail or vehicle. After 4-weeks, all mice were administered one ɸCT dose. Spleens were harvested 24 h after single injection and tested for presence of phage using the spot titer method. Data are representative of three experiments with n = 5 per group. For (F), Mann–Whitney test was used to compare groups. Medians with interquartile ranges are shown. Significance indicated by *p < 0.01 **p < 0.001 ***p < 0.0001. Source data are provided as a Source Data file.
