Fig. 4: Identification of the phage protein targets of anti-phage antibodies. | Nature Communications

Fig. 4: Identification of the phage protein targets of anti-phage antibodies.

From: Phage-specific immunity impairs efficacy of bacteriophage targeting Vancomycin Resistant Enterococcus in a murine model

Fig. 4

A TEM images of IgG immunogold-labeled phages incubated with IgG from mice treated with ɸCT or vehicle control. Arrows refer to gold staining attachment points. B Detection of phage proteins targeted by antibodies using serum from mice treated with ɸCT, ɸ45, or vehicle control to probe whole phage proteins. L = Ladder. C Coomassie-stained SDS-page gel loaded with purified ɸ45 and ɸ53 with protein bands targeted by anti-phage antibodies numbered. Table 2 identifies number-labeled protein bands. Data are representative of two replicates with serum or IgG from combined n = 5 per group. L = Ladder. Source data are provided in Supplementary Fig. 6.

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