Table 2 Identification of phage proteins targeted by anti-phage antibodies

From: Phage-specific immunity impairs efficacy of bacteriophage targeting Vancomycin Resistant Enterococcus in a murine model

 

Mass spec results

BLAST results

Phage

Band

Coverage (%)

MW [kDA]

Description

% Identity

Accession

45

1

68

150.9

phage tail fiber protein

97.67

YP_009604020.1

45

2

78

121.1

phage minor structural protein

92.19

YP_009604019.1

45

3

80

57.9

portal protein

99.61

QEM41711.1

53

1

67

128.7

putative adsorption associated tail protein

99.91

AZU99954.1

53

2

40

203.7

structural component of the tail fiber

99.89

YP_010115135.1

53

3

99

61.9

tail sheath protein

99.82

YP_009219871.1

53

4

97

51.3

major capsid protein

100

YP_009219879.2

53

5

91

51.3

major capsid protein

100

YP_009219879.2

  1. Protein bands labeled in Fig. 4B were cut and analyzed using mass spectroscopy. A custom database of translated coding sequences generated from whole genome sequencing of these phage was used to identify phage protein.