Fig. 3: Swapping ECL3 on adenosine receptor subtypes.
From: Cryo-EM structures of adenosine receptor A3AR bound to selective agonists
a sequence alignment of ECL3 among adenosine receptors. The disulfide bond was shown as green linker. b Superimposed structures of adenosine receptors reveal that A3AR has the shortest ECL3. The residues in A3AR are shown in pink. The residues formed disulfide bond on ECL3 in A1AR were shown in green. Other TMs were omitted. c–e Assessing the effects of adenosine, CF101, and CF102 on A1AR, A2AAR, and A2BAR, along with their corresponding mutants containing the swapped ECL3 from the A3AR using NanoBiT assays. The results were from three independent experiments. Data shown are mean ± S.E.M. of three independent experiments (n = 3). Source data are provided as a Source Data file. f, g Assessing the effects of CF101 and CF102 on A3AR and its mutants with flexible ECL3 using NanoBiT assays. Data shown are mean ± S.E.M. of three independent experiments (n = 3). Source data are provided as a Source Data file.
