Fig. 2: Isolation and characterization of a panel of potent neutralizing antibodies through affinity maturation. | Nature Communications

Fig. 2: Isolation and characterization of a panel of potent neutralizing antibodies through affinity maturation.

From: Potent human neutralizing antibodies against Nipah virus derived from two ancestral antibody heavy chains

Fig. 2

a Neutralization profiles of the authentic henipavirus in Vero E6 cells for antibodies. The mixtures of virus and serially diluted antibodies were added to cells. After 72 h incubation, IC50 were calculated by fitting the number of plaques with serially diluted antibody to a sigmoidal dose-response curve. Data are represented as the mean ± S.D. from n = 3 biologically independent experiments. b Summary of the IC50 with 95% confidence intervals of antibodies evaluated by the plaque reduction neutralization titer (PRNT). >5 represents that the antibody has not reached its maximum neutralization effect at the highest dilution concentration. c Affinity measurement of antibodies for binding to immobilized NiV-RBP and HeV-RBP, measured by using BLI. The biosensors loaded with Fab fragments (NiV42 or 42-27) or RBPs were shown in Supplementary Fig. 2a. Source data are provided as a Source Data file.

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