Fig. 3: Comparison of the binding site of candidate antibodies and EphrinB2. | Nature Communications

Fig. 3: Comparison of the binding site of candidate antibodies and EphrinB2.

From: Potent human neutralizing antibodies against Nipah virus derived from two ancestral antibody heavy chains

Fig. 3

a, b ELISA-based epitope mapping of mAbs with alanine scanned mutants. Utilizing ELISA, 32 mutants of NiV-RBP and 29 mutants of HeV-RBP were employed to evaluate the capacity of antibodies to bind, and the median effective concentration (EC50) was calculated by fitting to a four-parameter curve using Prism software. Heatmap shows the fold-change in EC50 binding titers of antibodies binding to henipavirus RBPs alanine mutants of compared with the wild-type proteins. The results are color-coded according to the ranges listed (also see Supplementary Fig. 2b, c). Source data are provided as a Source Data file.

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