Fig. 5: TLS preserves the PGC developmental programme.
From: Primordial germ cell DNA demethylation and development require DNA translesion synthesis
a RT-qPCR expression analysis of PGCs from E12.5 embryos (n = 3 embryos per genotype. Data represent mean and s.d. P values were calculated by a two-tailed Mann–Whitney U-test). b RT-qPCR expression analysis of PGCs from E12.5 embryos. (Wildtype n = 6; PcnaR/R n = 7. Except for Hormad1 and Brdt, wildtype n = 3; PcnaR/R n = 5). c Box plot of global DNA CpG methylation levels in E6.5 epiblast cells and E12.5 PGCs. The center displays the median, boxes the interquartile range and whiskers the minimum and maximum of CpG methylation distribution of the genome in 5Kbp genomic windows (n = 513027, 318836, 498368). d Circos-plot representation of DNA methylation levels in E6.5 epiblast and E12.5 wildtype PGCs and E12.5 PcnaR/R PGCs. CpG methylation was averaged in 5 Mbp genomic windows and the average DNA methylation is represented as a histogram track. e Heatmap showing methylation levels for E6.5 epiblast and E12.5 PGCs. f Unclustered methylation heatmap of CpG methylation in E6.5 epiblast and E12.5 PGCs. g Bisulfite sequencing and quantification of the Line-1 element from E12.5 wildtype, PcnaR/R and Rev1-/- embryos (filled:methylated CpG, open:unmethylated CpG. Each point represents one embryo, data represent mean and s.d., P values were calculated by a two-tailed Mann–Whitney U-test). h Violin plots reflecting the DNA methylation levels of GRR gene bodies in E6.5 epiblast cells from wildtype embryos, E12.5 wildtype PGCs and E12.5 PcnaR/R PGCs. The center displays the median, boxes the interquartile range and whiskers the minimum and maximum of percentage methylation calculated over each gene body with each point representing an individual gene (n = 37, 37, 35 left to right). i CpG methylation across selected GRR genes in E6.5 epiblast and E12.5 PGCs. The plots represent the distribution of CpG methylation across genes segmented in 0.1 Kbp genomic windows. j RT-qPCR expression analysis of Tet1 and Dnmt1 in PGCs from E12.5 embryos. (For Tet1, n = 5 for both genotypes. Dnmt1, wildtype n = 6 and PcnaR/R n = 7. Data represent mean and s.d., P values were calculated by a two-tailed Mann–Whitney U-test).
