Fig. 7: Activated p41Plk3 suppressed PDAC tumorigenesis and metastasis.
a Left, pancreatic tissues or tumors removed on day 40 from nude mice (n = 5 mice/group) orthotopically injected with PATC148 cells (1 × 105 cells) harboring indicated p72Plk3, NRDC, and mutants. Right, tumor weight analysis. b, c Growth curve (b) and flow cytometry analysis (c) in PATC148 cells with inducible expression of p72Plk3 and p41Plk3 under the suspension culture at the indicated times. d Left, pancreatic tissues or tumors removed on day 53 from nude mice (n = 5) orthotopically injected with PATC148 cells (1 × 105 cells) with inducible expression of p72Plk3 or p41Plk3. Right, tumor weight analysis. e, f The rates of tumor formation, invasion, or metastasis (e) and hematoxylin and eosin stains of tissues and lesions (f) of the indicated groups in d. Arrowheads indicate liver or lung metastasis; arrow shows liver invasion. Bar, 100 µm. a, d–f +, on: mice were fed with Dox-containing water upon cell inoculation and continued for the indicated times. -, off: mice were maintained Dox-free. g Kaplan-Meier analysis of mice with AsPC-1 cells that grew for 2 weeks in a subcutaneous xenograft, after which liposome-mediated gene delivery of the indicated expression vectors was performed. VCTL, vector control (n = 5 mice/group). p value was calculated by Log-rank test. h The proposed working model of Plk3 and Plk1 activation. Error bars, Mean ± SD (a, d) or ± SEM (b, c), n = 3 independent experiments (b, c), two-tailed unpaired t-test (a–d). Source data are provided as a Source Data file.
