Fig. 1: Histological characterization of Split^Cre-ReaChR mice.

A–C RNAscope of Eyfp (ReaChR) with Ret, Calb, or Ntrk2 using DRG section of Split^Cre-ReaChR mice. White arrows and arrow heads indicate some overlapped and non-overlapped Eyfp⁺ neurons, respectively. The zoomed image of (C) shows high expression of Ntrk2 in some DRG neurons and satellite glial cells surrounding Ntrk2 negative neurons (marked by asterisk). Scale bar = 50 μm in all micrographs until otherwise mentioned. D Quantification of the percentage of Eyfp⁺ neurons expressing different markers and vice versa. 4–5 sections/mouse, n = 3 mice. Error bars represent Mean ± S.E.M. E Triple immunostaining of a lumbar spinal cord section showing laminar segregation of GFP⁺ central terminals and CGRP⁺ or IB4⁺ nociceptor terminals. F Double immunostaining of lumbar spinal cord section showing GFP⁺ central terminals innervate deeper dorsal horn lamina (III-V), indicated by VGlut1⁺ staining. G–I Double immunostaining of plantar skin sections showing that GFP⁺ peripheral axons are NF200⁺ and innervate Meissner’s corpuscles (revealed by S100 staining) but not K8⁺ Merkel cells. White arrows indicate some Meissner’s corpuscles, while white arrowheads indicate Merkel cells. 6–8 sections/mouse, n = 4–6 mice. Source data are provided as a Source Data file.