Fig. 6: SplitCre-ReaChR+ Aβ-LTMRs in the hind paw glabrous skin fire more action potentials in response to mechanical forces after CFA treatment.
A Two sample traces showing mechanically evoked RA impulses recorded from light sensitive (ReaChR/YFP tagged) afferent fibers that innervate the glabrous skin of hind paws. Top, Saline-injected paw (control), Bottom, CFA-injected paw. RA impulses were evoked by tissue indentation at 30 mN at the receptive field where red LED light evoked impulses. Sample traces at expanded time scale show light-evoked (red) and mechanically evoked AP impulses (black). B Summary data of RA impulses evoked by tissue indentation at 0, 15, 30, and 80 mN in light-sensitive afferent fibers of saline-injected group (n = 15) and CFA-injected group (n = 5, p = 0.0016 at 80 mN). C Similar to (A) except SA impulses were recorded from a saline-injected paw (top, control) and CFA-injected paw (bottom). D Similar to (B) except SA impulses were recorded from the control group (n = 3) and CFA-injected group (n = 6). E Percent of light-sensitive afferent fibers that show RA (n = 19) or SA impulses in control group (n = 3), and RA (n = 5) or SA impulses (n = 6) in CFA group (p = 0.013), *p < 0.05, two-sided Chi’s square test. Grey and black bars show RA and SA recordings respectively. F Thresholds of RA-LTMRs in control (n = 19) and CFA (n = 5) groups. G Thresholds of SA-LTMRs in control (n = 3) and CFA (n = 6) groups. In (D, G), control group was pooled from both saline-injected and un-injected paws, 2 saline-injected and one un-injected paws for SA in (D, E, G); 15 saline-injected and 4 un-injected paws for RA in (E, F). In (B, D, F, G), data represent Mean ± S.E.M., **p < 0.01, two-way ANOVA with post hoc Bonferroni’s test, or ns, not significantly different, two-sided Student’s t-test. Source data are provided as a Source Data file.
