Fig. 2: LRP5-deficiency reduces PUFA accretion in neutrophils.

a Mice lacking LRP5 in neutrophils (Lrp5^N) and their corresponding wildtype (WT) littermate control mice on the normal diet (ND) or essential fatty acid-free diets (EFA) were subjected to myocardial ischemia-reperfusion injury. Infarction area (I) normalized against risk area (R) and Ejection Fractions before and after myocardial ischemia-reperfusion injury are shown. Each datum point represents one mouse (n = 8 for WT-ND(black circle), n = 7 for Lrp5^N-ND(blue square), n = 5 for WT-EFA(red circle) and n = 6 for Lrp5^N-EFA(green square)). Data were combined from three independent experiments. Data are presented as mean ± sem (One-way ANOVA for I/R and R/T, Two-way ANOVA for Ejection fraction, *p < 0.05). Volcano plots showing lipid species that are significantly (p < 0.05, Log₂Fold > 0.3 or <−0.3, Student’s t-test, Two-tailed, unpaired) increased (red) and decreased (blue) in LRP5-deficient neutrophils compared to corresponding WT cells from mice on normal diet (b) or EFA-free diet (c). Dark blue and dark red points are lipids containing PUFAs; light blue and light red points are lipids not containing PUFAs. The complete dataset is shown in Supplementary Data 1 and Data 2. d, f Neutrophils isolated from Lrp5^N (KO) or WT mice fed on the essential fatty acid-free diet were incubated with ¹⁴C-EPA in the presence and absence of BSA or ¹⁴C-ARA or ¹⁴C-oleic acid (OL) with BSA. The uptake by WT cells is taken as 100% (n = 3 per group in (d) and ARA group in (f), n = 5 per group in OL group in (f)). e Targeted LC-MS analysis of deuterated EPA in neutrophils (n = 3 per group). Data in (d–f) are presented as mean ± sem with p values (Student’s t-test, Two-tailed, unpaired).