Fig. 3: LRP5 deficiency reduces PUFAs uptake in various cell types. | Nature Communications

Fig. 3: LRP5 deficiency reduces PUFAs uptake in various cell types.

From: LDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments

Fig. 3

a 14C-EPA and 14C-ARA uptakes were determined using splenic NK cells isolated from NCR-Cre LRP5 KO and WT littermates, peritoneal macrophages isolated from Lrp5M and littermate WT mice, naïve splenic T lymphocytes, B lymphocytes, pulmonary fibroblast cells, and pulmonary endothelial cells prepared from Rosa-CreER2-driven LRP5 conditional KO and littermate WT treated with tamoxifen (n = 3 per group). b 14C-EPA uptake was determined using LRP5 KO HEK293T cells and WT cells (n = 5 per group). LRP5 KO HEK293T cells were generated by CRISPR/Case9 and validated by Western analysis. Data in this figure are all presented as mean ± sem with p values (Student’s t-test, Two-tailed, unpaired).

Back to article page