Fig. 4: LRP5 LDLa repeats bind to PUFAs. | Nature Communications

Fig. 4: LRP5 LDLa repeats bind to PUFAs.

From: LDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments

Fig. 4

a, b Binding of LRP5 LDLa repeats to fatty acids was determined by coating the plate with BSA or BSA plus the indicated fatty acids, followed by incubation with conditioned medium from cells expressing Fc or LRP5 LDLa-Fc. The binding of Fc to BSA is set as 1 (n = 4 per group). c Binding of purified LRP5 LDLa repeats fused with Fc to various fatty acids (non-linear fitting with Hill slope, n = 3 per group). Binding of these fatty acids to Fc was subtracted. AU, arbitrary unit. d Binding of purified LRP5 LDLa repeats fused with Fc to EPA or EPA ethyl ester (EPA-EE) (n = 8 per group). Data in this figure are all presented as mean ± sem with p values (Student’s t-test, Two-tailed, unpaired).

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