Fig. 6: LRP5 is required for n-3 PUFA-mediated mTORC1 suppression in neutrophils. | Nature Communications

Fig. 6: LRP5 is required for n-3 PUFA-mediated mTORC1 suppression in neutrophils.

From: LDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments

Fig. 6

Western analysis of WT and LRP5 KO neutrophils isolated from peritonea pretreated with thioglycolate for 12 h (a) or bone marrow neutrophils stimulated with fMLP (1 µM, (b)). Western qualification was normalized to corresponding total proteins (n = 3 per group from three independent experiments). c Bone marrow neutrophils isolated from WT and neutrophil-specific LRP5 KO mice fed on the normal diet or essential fatty acid-free diet with or without gavage of EPA were analyzed by Western blotting. Western blot qualification was normalized to HSP90 (n = 3 per group from three independent experiments). Data are presented as mean ± sem with p values (Student’s t-test, Two-tailed, unpaired for (a, b); One-way ANOVA for (c)). d The mTORC1 complex was immunoprecipitated from HEK293T cells expressing HA-tagged Raptor via an anti-HA antibody and subjected to in vitro kinase assay with recombinant 4EBP1 as the substrate in the presence or absence of ATP, EPA, and EPA-EE. e The phosphorylation of 4EBP1 was quantified with normalization against total 4EBP1 (n = 3 per group from three independent experiments). Data are presented as mean ± sem with p values (One-way ANOVA).

Back to article page