Fig. 2: Hepatoblastoma cells display a continuum of states between 3 differentiation poles.

a Scree plot showing the standard deviation of principal components (PCs) in the snRNA-seq data of tumor cells. b Projection of tumor cells from the 6 HB samples on the 2 first principal components. Tumor cells are colored by sample. Density plots show the distribution of cells from each sample along the two PCA axes. c Mean expression of Hepatocytic, Liver Progenitor and Mesenchymal signatures from bulk RNA-seq⁵ are shown onto the snRNA-seq PCA of tumor cells. d Single-cell states defined using thresholds on PC1 and PC2 include 3 poles (scH, scLP and scM) and one intermediate state (scH/LP). e Expression of representative markers of single-cell states (top). SLCO1B1, FRAS1 and COL5A1 are markers of the scH, scLP and scM state, respectively. APOB is expressed in all epithelial cells (scH, scH/LP and scLP). ATAC-seq tracks below indicate chromatin accessibility at the gene promoter in each cell state. f Proportion of single-cell states in each tumor. The molecular group of the matched bulk RNA-seq sample is indicated below. g snRNA-seq UMAP of all tumor cells colored by single-cell state. h snATAC-seq UMAP of all tumor cells (that passed snRNA-seq and snATAC-seq QC) colored by single-cell state. Source data are provided in the Source Data file.