Fig. 1: Quantitative HOIP interactome profiles in mouse tissues. | Nature Communications

Fig. 1: Quantitative HOIP interactome profiles in mouse tissues.

From: Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Fig. 1

a Workflow used to generate the four HOIP domains interaction network in nine tissues. This includes 45 GST pull-downs, triplicate runs on a hybrid linear ion trap-Orbitrap mass spectrometer. b Protein-protein interactions (PPIs) network of four HOIP domains across nine tissues. Preys are shown as squares and tissue-specific preys are indicated by colors. c Numbers of HOIP PPIs quantified across tissues. d Numbers of HOIP PPIs quantified across domains. e Numbers of all HOIP PPIs detected in different Affinity purification coupled to mass spectrometry (AP-MS) studies that are also reported by additional studies deposited in the BioGRID database. f Representative HOIP PPIs that are recapitulated in the generated network. g Dot plot of changes of HOIP interacting proteins STAT1 and β-Catenin across tissues and domains. Size of dot represents protein abundance relative to GST. Color of dot represents p value between domain and GST. p values are from the Limma moderated t-test. Immunoprecipitation analysis of known interacting proteins, such as STAT1 (h) and β-Catenin (i) in domains and tissues. The GST alone protein was a negative control. Source data are provided as a Source Data file.

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