Fig. 3: Gadd45g deletions induce MPN in mice after 10 months of age.

a Kaplan-Meier survival curves of Gadd45g^+/−, Gadd45g^−/− and Ctrl mice (n = 27-30 mice per group, log-rank test). b Counts of RBC, hemoglobin, WBC, platelet, and MCV, and percentages of monocytes, neutrophils and lymphocytes in the PB of moribund Gadd45g-insufficient mice with MPN (n = 25) and Ctrl mice (n = 10). c Wright-Giemsa staining of PB smears prepared from Ctrl and mice with MPN. Red arrowheads indicate abundant platelets. Bar represents 50 μm. At least three independent biological replicates were performed. d Total number of BM cells in moribund mice with MPN (n = 25) and Ctrl mice (n = 10). e Spleen weights and representative images of spleens of moribund mice with MPN (n = 25) and Ctrl mice (n = 10). f Liver weights and representative images of livers of moribund mice with MPN (n = 25) and Ctrl mice (n = 10). g H&E staining of femur (top) and spleen (bottom) sections from Ctrl and moribund mice with MPN. Yellow arrowheads indicate megakaryocytes. Bars represent 100 μm. At least three independent biological replicates were performed. h–n Absolute number of CD41⁺CD61⁺ (h), Gr-1⁺(i), Mac-1⁺(j), Gr-1⁺Mac-1⁺ (k), Ter119⁺ (l), GMP (m), MEP (n) cells in the BM of moribund mice with MPN (n = 25) and Ctrl mice (n = 10). For (b, d–f, h–n): Data are shown as means ± SD. Two-tailed Student’s t test.