Fig. 1: Glucose starvation stress granules are present in the bloodstream form of T. brucei and their formation is independent of the presence and activity of TbDYRK in vitro. | Nature Communications

Fig. 1: Glucose starvation stress granules are present in the bloodstream form of T. brucei and their formation is independent of the presence and activity of TbDYRK in vitro.

From: Differentiation granules, a dynamic regulator of T. brucei development

Fig. 1

Immunofluorescence of bloodstream slender cells exposed to glucose starvation for 30 min and 60 min in respective cell lines: (A) Parental = AnTat1.1 J1339, (B) TbDYRK-/- = AnTat1.1 J1339 TbDYRK-/-, (C) TbDYRK-/- Add-back NM = AnTat1.1 J1339 TbDYRK-/+(add-back; non mutated), (D) TbDYRK-/- Add-back S856G = AnTat1.1 J1339 TbDYRK-/+(add-back mutant S856G). Alba 3/4 localisation is revealed by anti-Alba 3 antibody (ab) (false coloured yellow), nucleus and kinetoplast using DAPI (false coloured blue). Scale bar = 10 μm. n = 3 independent experiments with similar outcome and no results were excluded.

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