Fig. 2: TbDYRK controls glucose stress granule dynamism.

Quantification of Alba 4+ve granules in the Wt and TbDYRK-/- cell lines during the recovery from glucose starvation condition. The non-parametric Wilcoxon test was used to compare means of two independent samples. The null hypothesis states that the distributions of both populations are identical. Assuming that the responses are continuous, the alternative is restricted to a shift in location. P values are indicated when the null hypothesis is rejected; NS non-significant compared to the +glucose condition in the respective cell line. A detailed significance table and the number of values measured can be found in supplementary data 1. Bloodstream slender cells were exposed to glucose starvation for 35 min (-gluc) followed by addition of glucose for 10 (10 min + Gluc), 30 (30 min + Gluc) and 60 min (60 min + Gluc). Cell line: Wt background = AnTat1.1 J1339 TbDYRK+/+ Alba 4::TYmNG, TbDYRK-/- background = AnTat1.1 J1339 TbDYRK-/- Alba 4::TYmNG. Alba 4 localisation in granules was revealed by anti-Ty antibody. The number of granules per cell was automatically evaluated using a script run in Fiji using the following parameters for the size = 0.12–1 µm and the circularity = 0.7–1. Boxplots represent the interquartile range (IQR) from the 1st (25th percentile, Q1) to the 3rd (75th percentile, Q3) quartile, the median and whiskers indicate the maximum (Q3 + 1.5*IQR) and minimum (Q1 − 1.5*IQR) values. Individual data points are shown using overlaid dot plots. Each dot represents one parasite. n = 3 biological replicates per n = 2 independent experiments were performed, no results were excluded.