Fig. 4: Localisation and composition of differentiation granules is dynamic during quorum-sensing differentiation.

A Quantification of Ty1-positive granules in the different cell lines during the time course of an infection at days D4, D5, D6 post-infection. n = 3 animals were infected with each cell lines over n = 3 independent experiments, no results were excluded. Boxplots represent the interquartile range (IQR) from the 1st (25th percentile, Q1) to the 3rd (75th percentile, Q3) quartile, the median and whiskers indicate the maximum (Q3 + 1.5*IQR) and minimum (Q1 − 1.5*IQR) values. Individual data points are shown using overlaid dot plots. Size of the dots represent the maximum of intensity measured in the corresponding cell. B Representation of Ty tag protein localisation. Diffuse - in granules either posterior to the nucleus, perinuclear or anterior to the nucleus—or in proximity to the STuRN/Flagellar pocket (FP), in relation to the distance between the kinetoplast and nucleus (KN distance in µm) in 1K1N cells—used to determine the relative stage of stumpy differentiation. Colours represent the time points in days post-infection (Days PI) and shapes represent the PAD1+ staining, being either negative (−ve, triangle) or positive (+ve, circle).