Fig. 5: Deletion of TbDYRK leads to uncontrolled accumulation of differentiation granules during quorum-sensing differentiation.
From: Differentiation granules, a dynamic regulator of T. brucei development
A Immunofluorescence of blood-smears of mice infected with Alba 4::APEX2-Flag tagged cell line, in TbDYRK+/+ (Wt) or TbDYRK-/- genetic background, at day 5 (D5) and day 6 (D6) post-infection. n = 3 animals were infected with each cell lines in n = 1 experiment. PAD1 expression is revealed using anti-PAD1 antibody (PAD1), Alba 4 localisation is revealed by anti-Flag antibody (Alba 4, false coloured Yellow), nucleus and kinetoplast using DAPI (false coloured Blue). PC Phase contrast. Acquisition settings were kept constant to detect weak positive cells, leading to saturation of the PAD1 signal on late time points. Scale bar = 10 μm. Arrows highlight differentiation granules. B Quantification of Flag positive granules in the different cell lines during the time course of the infection relative to the expression of the stumpy differentiation marker PAD1 negative (PAD1-ve) or positive (PAD1+ve). Boxplots represent the interquartile range (IQR) from the 1st (25th percentile, Q1) to the 3rd (75th percentile, Q3) quartile, the median and whiskers indicate the maximum (Q3 + 1.5*IQR) and minimum (Q1 − 1.5*IQR) values. Individual data points are shown using overlaid dot plots.
