Fig. 6: Dynamic compositions of starvation stress and differentiation granules are regulated by TbDYRK.

Circular plots representing proteins proximal to Alba 3 and Alba 4 in Wt background or Alba 4 in TbDYRK background identified by mass spectrometry. Circular axes represent the log2 fold change enrichment in the bait protein compared to the parental cell line. The y-axis is the -log10 of the p value. Statistical enrichment analysis was performed using two-sided moderated t-statistic as described in the Methods section. Proteins presenting a fold change >1.5 and a p < 0.05 in a given condition were considered statistically enriched. Statistically enriched proteins proximal to the bait protein are highlighted in yellow. A Stress granule composition in vitro in non-stress (top panels) or glucose starvation (bottom panels). Numbers of independent biological replicates used for analysis: Parental no stress n = 3, parental starvation n = 2, Alba 4 in Wt no stress n = 3 and starvation n = 3, Alba 4 in TbDYRK-/- no stress n = 2 and starvation n = 2. B Differentiation granule composition at two different time points during the course of mice infection, early (top panels) and late (bottom panels).