Fig. 1: Establishment and validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells.
From: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells
A Schematic of the CRISPR/Cas9 genome editing strategy to endogenously tag UHRF1 with mAID/mClover and DNMT1 with mAID/mRuby2. B Order of events for the generation of the different cell lines. C Immunoblot images for validation of endogenous AID-tagged UHRF1 and/or DNMT1 HCT116 cells. Experiments in each panel were performed at least three times, and the representative results are shown. D Representative fluorescence images on UHRF1-AID/DNMT1-AID HCT116 cells showing that tagged UHRF1 and DNMT1 co-localize. E Quantification of the DNA methylation level in each HCT116 cell line with LUMA, LC-MS/MS, or WGBS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (*p < 0.05). Data are presented as mean values +/− SEM from biological triplicates. Source data are provided as a Source Data file.
