Fig. 2: The depletion of UHRF1 and DNMT1 is efficient, negatively affects growth, and can be rescued genetically.
From: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells
A Immunoblot of HCT116 cells following treatment with Auxin (IAA) at the indicated time points (hours) and before treatment (NT). Experiments in each panel were performed at least three times, and the representative results are shown. B Cell proliferation of the HCT116 derivatives in the continuous presence of auxin for the indicated durations (Incucyte videomicroscopy). Error bars represent the SEM of biological triplicates. C Cell proliferation of the HCT116 derivatives in the continuous presence of auxin for the indicated durations (cell counting). The error bars represent the SEM of biological triplicates. D Schematic of the rescue experiments. E UHRF1 domain map showing the mutants studied (left panel) and corresponding cell proliferation analysis (Cell count, right panel). Error bars represent the SEM of three independent experiments. F Same as (E), but for DNMT1. Source data are provided as a Source data file.
