Fig. 5: Physical and functional interaction between UHRF1, DNMT3A, and DNMT3B.

A Western blotting after the indicated co-immunoprecipitation experiments. hUHRF1: Full-length protein. The other names indicate isolated domains, as depicted in Fig. 2E. B Same as in (A), except we used truncated constructs in which the indicated domains were deleted from the full-length protein. C Same as in (A), except we used a full-length UHRF1 protein with a point mutation in the Tandem Tudor Domain (Y188A/Y191A). D Western blotting showing abundance of the indicated proteins in total cell extracts. E Quantitation of the loss of DNA methylation in the indicated cell lines after 8 days of protein depletion, by LC-MS/MS. The p value is calculated with one-way ANOVA and Tukey’s HSD test (N.S. p > 0.05, **p < 0.01). Data are presented as mean values +/− SEM from biological triplicates. A–D Experiments in each panel were performed at least two times, and the representative results are shown. Source data are provided as a Source data file.