Fig. 6: UHRF1 protects against active demethylation by TET2.
From: Non-canonical functions of UHRF1 maintain DNA methylation homeostasis in cancer cells
A Workflow used to quantitatively compare WGBS methylation values to the in vitro preferences of TET1 and TET2. B RT-qPCR analysis for validation of TET2 knockdown for HCT116 UHRF1 and/or DNMT1-AID cell lines. C Global DNA methylation analysis (LUMA) for HCT116 UHRF1 and/or DNMT1-AID cell lines combined with TET2 knockdown. D LC-MS/MS analysis on the indicated samples. Error bars represent the SEM of three independent experiments. The p value is calculated using two-sided Student’s t test (N.S. p > 0.05, ***p < 0.001). Source data are provided as a Source data file.
