Fig. 3: Lovastatin via circRNA-RBCK1 suppresses the function of miR-133a in human endocardium endothelial cells (EEC). | Nature Communications

Fig. 3: Lovastatin via circRNA-RBCK1 suppresses the function of miR-133a in human endocardium endothelial cells (EEC).

From: Statins improve cardiac endothelial function to prevent heart failure with preserved ejection fraction through upregulating circRNA-RBCK1

Fig. 3

Human EECs infected with lentivirus harboring scramble shRNA or circRNA-RBCK1 shRNA for 48 h were incubated with ox-LDL (100 μg/ml) plus lovastatin (10 μM) for 24 h. Cells were harvested to assay (A) GTPCH1 mRNA by quantitative PCR, (B) BH4 contents by HPLC, and (C) ROS productions by DHE/HPLC, and (D) NO levels by DAF/HPLC. N = 5 per group. A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (A–D). Data are presented as mean ± SD. Source data are provided as a Source Data file.

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