Fig. 2: Reconstruction of a cellular atlas for mature pericarp of H. undatus using spatial transcriptomics.
From: Single-cell and spatial RNA sequencing reveal the spatiotemporal trajectories of fruit senescence
a Workflow for sampling and sequencing H. undatus pericarp on the 10× Visium platform. b Illustrations of cell types discovered on glass slides, overlaid on corresponding H&E-stained images. Clusters are named based on the spatial positioning of cell types. c Spatial localization maps (left) and violin plots (right) showing the expression of marker genes for different cell types. The violin plot showed six data nodes for each set of data, arranged from largest to smallest, namely the maximum value (upper edge), the upper quartile, the median, the lower quartile, and the minimum value (lower edge). The sample sizes (n number) are shown on each panel. d Association analysis between spatial transcriptomics and single-cell transcriptomics using four algorithms: SciBet, SingleR, RCTD, and CARD. e UMAP plot displaying 13 clusters of single cells classified into 5 cell types after spatial transcriptomics identification.
