Fig. 1: Framework to assess biomolecular condensates in bacteria.
From: An experimental framework to assess biomolecular condensates in bacteria
Proteins of interest or control proteins are inducibly expressed to form foci (dark purple circle) in E. coli (top). The in vivo saturation concentration (csaturation) is quantified. The material state of each focus can be assessed: (Left) Condensates dissolve upon cell growth, cell division, cell shape change, temperature shift, or osmolarity shift, whereas insoluble aggregates remain intact. (Middle) Analysis of protein dynamics informs on the material properties of the focus. Dashed magenta circles: photobleached areas. (Right) The chaperone IbpA (yellow) surrounds insoluble aggregates but penetrates condensates.
