Fig. 3: Cells that form condensates maintain a soluble cytoplasmic fraction.
From: An experimental framework to assess biomolecular condensates in bacteria
a Partition ratios for specified mCherry fusions with detected foci (cIagg, N = 995 cells; PopTagSL, N = 939 cells; PopTagLL, N = 147 cells; McdB, N = 341 cells; McdBsol, N = 714 cells). b Single-cell condensation coefficients for specified mCherry fusions with detected foci cIagg, N = 1019 cells; PopTagSL, N = 942 cells; PopTagLL, N = 151 cells; McdB, N = 343 cells; McdBsol, N = 717 cells. An intensity threshold I = 0.3 was used. Data points correspond to individual cells and were normalized the mean condensation coefficient of cells expressing mCherry only. The shaded region represents the measurement range for cells expressing a uniform mCherry signal. The curves next to the scatter plots were obtained via kernel density estimation. N values are the same as in (a). c Normalized single-molecule localization 2D histograms. Single-molecule localizations of specified PAmCherry fusion proteins were collected, projected, and binned onto a normalized cell shape to generate the heat map. Localizations were collected from 30 cells or more per condition over three biological replicates. *p < 0.05, **p < 0.01, and ***p < 0.001 by two-sided Welch’s t-test. Source data are provided as a Source Data file.
